Recombinant Dna Technology As An Environment For Separation

Recombinant DNA technology is genetic engineering process for forming a new gene. The gene required is taken from the donor and joined with the carrier gene which is then inserted into the vector. This method is used to create a vector containing gene from Bacteria sp Yp1 and Esterobacter asburae YT1, which are then inserted to egg through microinjection. Microinjection is a process where screw held syringe is loaded with required DNA or RNA and inserted to animal cell. By this technique the cloned gene is inserted to egg of earthworm. The egg hatchs and develops further producing transgenetic species having gene of gut bacteria. Sodium dodecyl sulphate PolyAcrylamide Gel Electroporesis (SDS-PAGE) is a technique used for separating protein based on their size and structure [14]. Sodium dodecyl sulphate (SDS) is an anionic agent applied on proteins for linearizing them and to impart negative charge on the proteins. When electric field is applied on protein covered with negative charge, they move towards positive pole but no size separation can be seen. So PolyAcylamide gel is used as an environment for separation. As electric potential is applied on proteins present in PAGE, it creates even distribution of charge per unit mass resulting in fractionation of protein based on their size[15]. SDS-PAGE is useful technique for acquiring the required protein. Once the transgenic Earthworm is created, this technique can be used to acquire the specific protein/enzyme responsible forShow MoreRelatedBiological Molecules Like Nucleic Acids And Polysachharides2245 Words   |  9 Pagessource. Then amplification of these i solated genes is done. Amplification is done by the insertion of the target gene into a specified vector. Vector is also a DNA sequence molecule. The vector that has foreign gene in now a recombinant DNA. It replicates in cells after insertion in living cells. 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